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patient derived breast cancer stem cells  (Celprogen Inc)


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    Celprogen Inc patient derived breast cancer stem cells
    GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of <t>cancer</t> cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer <t>cells/stem</t> cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. <t>Patient-derived</t> CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .
    Patient Derived Breast Cancer Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/patient derived breast cancer stem cells/product/Celprogen Inc
    Average 93 stars, based on 17 article reviews
    patient derived breast cancer stem cells - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Safe immunosuppression-resistant pan-cancer immunotherapeutics by velcro-like density-dependent targeting of tumor-associated carbohydrate antigens"

    Article Title: Safe immunosuppression-resistant pan-cancer immunotherapeutics by velcro-like density-dependent targeting of tumor-associated carbohydrate antigens

    Journal: Cell

    doi: 10.1016/j.cell.2025.09.001

    GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of cancer cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer cells/stem cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. Patient-derived CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .
    Figure Legend Snippet: GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of cancer cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer cells/stem cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. Patient-derived CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .

    Techniques Used: Flow Cytometry, Co-Culture Assay, Derivative Assay, Cell Culture, Injection, Binding Assay



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    GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of <t>cancer</t> cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer <t>cells/stem</t> cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. <t>Patient-derived</t> CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .
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    GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of cancer cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer cells/stem cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. Patient-derived CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .

    Journal: Cell

    Article Title: Safe immunosuppression-resistant pan-cancer immunotherapeutics by velcro-like density-dependent targeting of tumor-associated carbohydrate antigens

    doi: 10.1016/j.cell.2025.09.001

    Figure Lengend Snippet: GlyTR1 overcomes multiple immunosuppressive mechanisms in the tumor microenvironment (A–E) Flow cytometry of cancer cell death triggered by GlyTR1 or L-PHA following co-culture of the indicated cancer cells/stem cells with/without human CD8 + T cells under the indicated conditions for 3 (A–D) or 2 (E) days. Patient-derived CSCs (E) were CD133 + OCT4 + SSEA3/4 + and cultured in a hypoxic chamber (5% O2, 5% CO2, and 90% N2). Data are the mean ± SEM of 3 biological replicates. (F and G) The indicated solid tumors grown in NSG mice without (F) or with PBMC co-injection (G) were analyzed by flow cytometry for immune cells as indicated or embedded in an air-liquid interface (ALI) organoid culture system with (F) or without the addition of exogenous PBMC (G). Tumor fragments were embedded in a transwell with a porous bottom containing a type I-A collagen solid matrix exposed to air and placed in a 60 mm dish containing organoid culture media with or without 100 ng/mL (F) or 500 (G) ng/mL) GlyTR1. After 7–14 days of ALI culture, single-cell suspensions were analyzed for live tumor cells by flow cytometry. FVD, fixable viability dye eFluor ™ 780. (H) Fresh surgically resected vimentin + Ewing’s sarcoma metastatic to the lung was analyzed by flow cytometry for hCD45 + immune cells or GlyTR1 binding to cell-surface-vimentin + (CSV + ) tumor cells, and the latter was compared with MDA-MB-231F-MI − clone 11 TNBC. In parallel, an equal amount (by weight) of fresh tumor pieces was used in the ALI organoid system (as in G) and treated with or without GlyTR1 (500 ng/ml). After 6 days, the tumor was digested into single cells and plated/cultured overnight. Live adherent cells were imaged and counted. Flow cytometry confirmed adhered cells were CSV + . Data are the mean ± SEM of 3 biological replicates. See also and .

    Article Snippet: Patient derived Breast cancer stem cells , Celprogen Inc. , 36102-29.

    Techniques: Flow Cytometry, Co-Culture Assay, Derivative Assay, Cell Culture, Injection, Binding Assay